Monitoring Methods

Introduction

To establish a baseline using bioassay procedures, a reliable technique must be developed. The technique must also be able to be used in subsequent monitoring operations investigating possible development of resistance. If and when resistance develops, the same method may be used to identify resistant isolates, but it is possible that more specific methods will have been developed at that time.

In a joint effort to create standardized methods, members of different FRAC Working Groups have reviewed and collected detailed, ready-to-use bioassay techniques to monitor fungal pathogens of economic importance.

The following requirements were considered while establishing these techniques:

• The method must be robust, reliable and repeatable
• It must be as simple as possible to operate in terms of technology and user skills
• It should be as cheap to operate as possible and capable of a high throughput in a short time
• The data obtained must be able to be related to sensitivity responses in the field

In a joint effort to create standardized methods, members of different FRAC Working Groups have reviewed and collected detailed, ready-to-use bioassay techniques to monitor fungal pathogens of economic importance.

List of approved methods

Here is the list of FRAC approved methods. The assays are sorted by pathogen and assay type. Pathogens are listed by their approved EPPO code: