FRAC Monitoring Methods
Introduction

To establish a baseline using bioassay procedures, a reliable technique must be developed. The technique must also be able to be used in subsequent monitoring operations investigating possible development of resistance. If and when resistance develops, the same method may be used to identify resistant isolates, but it is possible that more specific methods will have been developed at that time.

In a joint effort to create standardized methods, members of different FRAC Working Groups have reviewed and collected detailed, ready-to-use bioassay techniques to monitor fungal pathogens of economic importance.

The following requirements where considered while establishing these techniques:

• The method must be robust, reliable and repeatable
• It must be as simple as possible to operate in terms of technology and user skills
• It should be as cheap to operate as possible and capable of a high throughput in a short time
• The data obtained must be able to be related to sensitivity responses in the field
 
   
List of approved methods

Here is the list of FRAC approved methods. The assays are sorted by pathogen and assay type. Pathogens are listed by their approved EPPO Code System.
 
List of Monitoring Methods (Status Jan. 2012)  
     
Approved Methods
   
ALTESO monitoring method Syngenta 2006 V1.pdf
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BOTRCI microtiter monitoring method BASF 2009 V2.pdf
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BOTRCI microtiter monitoring method BCS 2006 V1.pdf
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BOTRCI monitoring method Syngenta 2006 V1.pdf
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BOTRCI apple test monitoring method BCS 2006 V1.pdf

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ERYSGH leaf segment monitoring method EpiL. 2006 V1.pdf
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ERYSGT in vivo BASF 2006 V1.pdf
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ERYSGT leaf segment monitoring method EpiL. 2006 V1.pdf
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GIBBZE microtiter monitoring method BCS 2006 V1.pdf
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MONGNI microtiter monitoring method BCS 2006 V1.pdf
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MONISP germ tube elongation test BASF 2009 V1.pdf
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MYCOFI in vitro germ tube method Monreri 2008 V1.pdf
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MYCOFI in vitro germ tube method Pathotec 2008 V1.pdf
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MYCOFI in vitro germ tube method PBGEA 2008 V1.pdf
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PHAKPA detached leaf monitoring method BCS 2006 V1.pdf
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PHAKPA in vitro method Syngenta 2006 V1.pdf
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PHYTIN in vivo method BASF 2006 V1.pdf
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PHYTIN in vivo method Syngenta 2007 V1.pdf
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PHYTIN microtiter method sporangia BASF 2006 V1.pdf
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PHYTIN microtiter plate method DuPont 2006 V1.pdf
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PHYTIN tissue culture plate method BASF 2006 V1.pdf
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PLASVI microtiter monitoring method Syngenta 2003 V1.pdf
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PSDCHE microtiter monitoring method BASF 2009 V1.pdf
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PSDCHE microtiter monitoring method BCS 2006 V1.pdf
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PUCCRE leaf segment monitoring method EpiL. 2006 V1.pdf
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PYRNTE monitoring method BASF 2009 V1.pdf
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PYRNTE monitoring method Syngenta 2006 V1.pdf
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PYRNTR monitoring method Syngenta 2006 V1.pdf
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RHYNSE monitoring method Syngenta 2006 V1.pdf
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SCLESC tissue culture plate method BASF 2008 V1.pdf
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SEPTTR microtiter monitoring method BASF 2009 V1.pdf
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SEPTTR microtiter monitoring method BCS 2006 V1.pdf
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SEPTTR monitoring method Syngenta 2006 V1.pdf
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SEPTTR radial growth BCS 2006 V1.pdf
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UNCINE leaf disc monitoring method EpiL. 2006 V1.pdf
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VENTIN in vivo-AP BASF 2006 V1.pdf
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VENTIN monitoring method Syngenta 2006 V1.pdf
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VENTIN spore germination BASF 2006 V1.pdf
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Status: February 2009